Major Characters
| Kenshin Himura | Kaoru Kamiya | Yahiko Myoujin |
| Sanosuke Sagara | Megumi Takani | Misao Makamachi |
| Aoshi Shinomori | Hajime Saito |
Supporting Characters
| Seijouro Hiko | Makoto Shishio | Sojiro Seta |
| Yumi Komagato | Dr. Gensai | Okina |
| Ayame and Suzume | Tai | Tsubame |
| Juppongatana | Oniwaban Group | Captain Sagara |
| Yutaro |